Tuesday, January 18, 2011

Leech Nuerophysiology Lab

Purpose:  The purpose of this lab is to record the electrical activity found in the neurons of the leech and to identify which neurons respond to certain stimuli.
Hypothesis:  Considering the leeches nervous system.  the electrical activity would be easy to identify in the ganglia because they are so large and serve the same purpose.
Materials:  Leech tank, dissection tray, 20% ethanol, dissecting microscope, micro manipulator, probe, forceps, scissors, dissection pins, leech tongs, Oscilloscope   
Procedure:  1. Anesthetize and dissect the leech 2. remove leech innards and observe ganglion 3. cut our the ganglion window 4. isolate one ganglion 5. cut the ganglion sinus 6. probe and identify ganglion sensory cells
Results and Conlusion: There was five cells found with different stimuli.  Basically, one ganglia contains many cells which contribute to the whole nervous system.